Improved Fractionation of Glycinin and B-Conglycinin and Partitioning of Phytochemicals

نویسندگان

  • David A. Rickert
  • Lawrence A. Johnson
  • Patricia A. Murphy
  • DAVID A. RICKERT
  • LAWRENCE A. JOHNSON
  • PATRICIA A. MURPHY
چکیده

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Improved fractionation of glycinin and beta-conglycinin and partitioning of phytochemicals.

Glycinin-rich and beta-conglycinin-rich products are prepared by soy protein fractionation. Physicochemical characteristics of these proteins affect their unique, important functionality in food systems and industrial applications. Soybean isoflavones and saponins are phytochemicals with potential health benefits. Objectives of this protein fractionation research were to (1) improve protein and...

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Identification of glycinin and beta-conglycinin subunits that contribute to the increased protein content of high-protein soybean lines.

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Differential Proteolysis of Glycinin and beta-Conglycinin Polypeptides during Soybean Germination and Seedling Growth.

The degradation of the major seed storage globulins of the soybean (Glycine max [L.] Merrill) was examined during the first 12 days of germination and seedling growth. The appearance of glycinin and beta-conglycinin degradation products was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cotyledon extracts followed by electroblotting to nitrocellulose and immunostaining...

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Improvement of the Nutritional Value of Soybean [Glycine max (L) Merr.] Seed with Alteration in Protein Subunits of Glycinin (11S Globulin) and β-conglycinin (7S Globulin)

Protein quality in soybean seeds is strongly influenced by nutritional conditions. Glycinin (11S globulin) and β-conglycinin (7S globulin) are the 2 main proteins in soybean seeds. Protein quality of glycinin (11S) is higher than that of β-conglycinin (7S), due to the presence of higher amounts of s-containing amino acids (methionine and cysteine) in glycinin than β-conglycinin. Of the amino ac...

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Bioactive proteins against pathogenic and spoilage bacteria

Glycinin, basic subunit and β-conglycinin were isolated from soybean protein isolate and tested for their antimicrobial action against pathogenic and spoilage bacteria as compared to penicillin. The three fractions exhibited antibacterial activities equivalent to or higher than penicillin in the next order; basic subunit>glycinin> β-conglycinin with MIC of 50, 100 and 1000 μg/mL, respectively. ...

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تاریخ انتشار 2017